Argonaute proteins have established themselves as key players in the RNAi mechanisms across all domains of life. In plants several types of argonaute proteins are present which are involved in the different small RNA related pathways. Likewise in animals, fungi (Quelling) and Protists argonaute proteins are mostly involved in the microRNA and siRNA related pathways. In some higher eukaryotic taxa they serve as elongation factors as well. This study explores the sequence complexities of Argonaute 1 protein across various domains of life and attempts to identify whether any intrinsically unstructured regions are present in the proteins under study.