In this study Green Tea Leaf Extract (GTLE) is used as a herbal agent for application on animals for 26 consecutive days. This extract was prepared following the method of Wei et al (1999). The extract was administered to two different experimental animal groups with two different doses. After treatment, it was found that normal weight gains of all the animals were taken place but the percentage was slightly high in control animal group compared to two other experimental groups. Weight of the testis and epididymis were decreased after administration of GTLE compared to the control group of animals. SGPT and SGOT were not altered significantly after application of GTLE. Sperm count was reduced in experimental animal groups significantly in dose dependent manner. The testosterone level was also decreased in two treatment groups in dose dependent manner comparing to the animals of controlled groups.